Publications of Ron Duggleby

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[1975-79] [1980-84] [1985-89] [1990-94] [1995] [1996] [1997] [1998] [1999] [2000] [2001] [2002] [2003] [2004] [2005] [2006 onwards]

  1. Chapman, P.J. and Duggleby, R.G. (1967) Dicarboxylic acid catabolism by bacteria. Biochem. J., 103: 7c-9c. View PDF file

  2. Macrae, A.R. and Duggleby, R.G. (1968) Substrates and inhibitors of potato tuber phenolase. Phytochem., 7: 855-861. View PDF file

  3. Duggleby, R.G. and Dennis, D.T. (1970) Nicotinamide adenine dinucleotide specific isocitrate dehydrogenase from a higher plant. The requirement for free and metal complexed isocitrate. J. Biol. Chem., 245: 3745-3750. View PDF file

  4. Duggleby, R.G. and Dennis, D.T. (1970) Regulation of nicotinamide adenine dinucleotide specific isocitrate dehydrogenase from a higher plant. The effect of reduced nicotinamide adenine dinucleotide and mixtures of citrate and isocitrate. J. Biol. Chem., 245: 3751-3754. View PDF file

  5. Duggleby, R.G. and Dennis, D.T. (1973) The characterisation and regulatory properties of pyruvate kinase from cotton seeds. Arch. Biochem. Biophys., 155: 270-277. View PDF file

  6. Duggleby, R.G. and Dennis, D.T. (1973) Pyruvate kinase, a possible regulatory enzyme in higher plants. Plant Physiol., 52: 312-317. View PDF file

  7. Duggleby, R.G. and Dennis, D.T. (1974) Nicotinamide adenine dinucleotide specific glyceraldehyde-3-phosphate dehydrogenase from Pisum sativum. Purification and characterization. J. Biol. Chem., 249: 162-166. View PDF file

  8. Duggleby, R.G. and Dennis, D.T. (1974) Nicotinamide adenine dinucleotide specific glyceraldehyde-3-phosphate dehydrogenase from Pisum sativum. Assay and steady state kinetics. J. Biol. Chem., 249: 167-174. View PDF file

  9. Duggleby, R.G. and Dennis, D.T. (1974) Nicotinamide adenine dinucleotide specific glyceraldehyde-3-phosphate dehydrogenase from Pisum sativum. Effect of nicotinamide adenine dinucleotide and related compounds on the enzyme-catalysed arsenolysis of 1,3-diphosphoglyceric acid. J. Biol. Chem., 249: 175-181. View PDF file

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  10. Duggleby, R.G. and Kaplan, H. (1975) A general method for the determination of the carboxyl-terminal sequence of proteins. Anal. Biochem., 65: 346-354. View PDF file

  11. Duggleby, R.G., Kaplan, H. and Visentin, L.P. (1975) Carboxyl-terminal sequences in procaryotic ribosomal proteins from Escherichia coli, Bacillus stearothermophilus, and Halobacterium cutirubrum. Can. J. Biochem., 53: 827-833.

  12. Duggleby, R.G. and Kaplan, H. (1975) A competitive labelling method for the determination of the chemical properties of solitary functional groups in proteins. Biochemistry, 14: 5168-5175.

  13. Kaplan, H. and Duggleby, R.G. (1975) Application of competitive labelling to the study of the effect of the environment on the chemical properties of groups in proteins: environmental effects on histidine residues. In Environmental effects on molecular structure and properties. Reidel Publishing Co., Boston: 409-417.

  14. Duggleby, R.G. and Morrison, J.F. (1977) The analysis of progress curves for enzyme-catalysed reactions by non-linear regression. Biochim. Biophys. Acta, 481: 297-312.

  15. Degani, Y., Duggleby, R.G. , Nyc, J.F. and Smith, E.L. (1977) Nicotinamide adenine dinucleotide-specific glutamate dehydrogenase of Neurospora. Tryptic peptides. J. Biol. Chem., 252: 8150-8159. View PDF file

  16. Ellis, K.J. and Duggleby, R.G. (1978) What happens when data are fitted to the wrong equation? Biochem. J., 171: 513-517. View PDF file

  17. Duggleby, R.G., Sneddon, M.K. and Morrison, J.F. (1978) Chorismate mutase-prephenate dehydratase from Escherichia coli: the active sites of a bifunctional enzyme. Biochemistry, 17: 1548-1554.

  18. Duggleby, R.G. and Morrison, J.F. (1978) Progress curve analysis in enzyme kinetics: model discrimination and parameter estimation. Biochim. Biophys. Acta, 526: 398-409.

  19. Duggleby, R.G. and Morrison, J.F. (1979) The use of steady-state rate equations to analyze progress curve data. Biochim. Biophys. Acta, 568: 357-362.

  20. Duggleby, R.G. (1979) A comment on the "jack-knife" technique for analysing enzyme-kinetic data. Biochem. J., 181: 255-256.

  21. Williams, J.W., Morrison, J.F. and Duggleby, R.G. (1979) Methotrexate, a high-affinity pseudo-substrate of dihydrofolate reductase. Biochemistry, 18: 2567-2573.

  22. Duggleby, R.G. (1979) Experimental designs for estimating the kinetic parameters for enzyme-catalysed reactions. J. Theor. Biol., 81: 671-684.

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  23. Williams, J.W., Duggleby, R.G., Cutler, R. and Morrison, J.F. (1980) The inhibition of dihydrofolate reductase by folate analogues: structural requirements or slow and tight-binding inhibition. Biochem. Pharmac., 29: 589-595.

  24. Duggleby, R.G. (1980) Estimation of the reliability of parameters obtained by non-linear regression. Eur. J. Biochem., 109: 93-96. View PDF file.

  25. Reilly, P.E.B. O'Shannessy, D.J. and Duggleby, R.G. (1980) Non-Michaelian monooxygenase kinetics: studies using competitive inhibitors. FEBS lett., 119: 63-67.

  26. Duggleby, R.G. (1981) A non-linear regression program for small computers. Anal. Biochem., 110: 9-18.

  27. Duggleby, R.G., Kinns, H. and Rood, J.I. (1981) A computer program for determining the size of DNA restriction fragments. Anal. Biochem., 110: 49-55.

  28. Duggleby, R.G. (1981) Experimental designs for the distribution-free analysis of enzyme kinetic data. In Kinetic data analysis: design and a nalysis of enzyme and pharmacokinetic experiments, Ed. L. Endrenyi. Plenum Press, New York: 169-179.

  29. Duggleby, R.G. (1981) A paucity of palindromes in phiX174. J. Theor. Biol., 93: 143-155.

  30. Duggleby, R.G. and Dennis, D.T. (1982) Glyceraldehyde-3-phosphate dehydrogenase from pea seeds. Meth. Enzymol., 89: 319-325.

  31. Duggleby, R.G. (1982) Application of nonlinear regression to the analysis of histidine transport by S37 ascites tumour cells. J. Theor. Biol., 95: 823-828.

  32. Duggleby, R.G., Attwood, P.V., Wallace, J.C. and Keech, D.B. (1982) Avidin is a slow-binding inhibitor of pyruvate carboxylase. Biochemistry, 21: 3364-3370.

  33. Duggleby, R.G. (1983) Determination of the kinetic properties of enzymes catalysing coupled reaction sequences. Biochim. Biophys. Acta, 744: 249-259.

  34. Christopherson, R.I. and Duggleby, R.G. (1983) Metabolic resistance: the protection of enzymes against drugs which are tight-binding inhibitors by the accumulation of substrate. Eur. J. Biochem., 134: 331-335. View PDF file.

  35. Duggleby, R.G. (1984) Regression analysis of nonlinear Arrhenius plots: an empirical model and a computer program. Comput. Biol. Med., 14: 447-455.

  36. Duggleby, R.G. and Christopherson, R.I. (1984) Metabolic resistance to tight-binding inhibitors of enzymes involved in the de novo pyrimidine pathway: simulation of time-dependent effects. Eur. J. Biochem., 143: 221-226. View PDF file.

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  37. Duggleby, R.G. (1985) QUTOR: a computer program for presenting tutorials. Biochem. Educat., 13: 59-61. View PDF file.

  38. Duggleby, R.G. (1985) Estimation of the initial velocity of enzyme-catalysed reactions by nonlinear regression analysis of progress curves. Biochem. J., 228: 55-60.

  39. Duggleby, R.G. (1985) Preparation of concentrated solutions of some common solutes by means of calculated molalities. Comput. Biol. Med. 15: 375-379.

  40. Duggleby, R.G. (1986) Progress curve analysis in enzyme kinetics. Numerical solution of integrated rate equations. Biochem. J., 235: 613-615.

  41. Duggleby, R.G. (1986) Progress curves of reactions catalyzed by unstable enzymes. A theoretical approach. J. Theor. Biol., 123: 67-80.

  42. Pike, S.J. and Duggleby, R.G. (1987) Resistance to inactivation by EGTA of the enzyme-substrate and enzyme-phosphate complexes of alkaline phosphatase. Biochem. J., 244: 781-785.

  43. Northrop, D.B. and Duggleby, R.G. (1987) Preparation and storage of isotopically-labelled reduced nicotinamide adenine dinucleotide. Anal. Biochem., 165: 362-364.

  44. Duggleby, R.G. (1988) Fitting the double Michaelis-Menten equation to kinetic data. J. Theor. Biol., 130: 123-124.

  45. Atukorala, T.M.S., Duggleby, R.G. and Nixon, P.F. (1988) Effects of acetaldehyde upon catalysis by human erythrocyte transketolase. Biochem. Pharmac., 37: 2100-2101.

  46. Duggleby, R.G. and Ward, L.C. (1988) Procedures and a computer program for the determination of fractional protein synthetic rates by numerical solution of an implicit equation. Comput. Biol. Med., 18: 245-251.

  47. Duggleby, R.G. (1988) The application of metabolic resistance theory to the selection of preferred target enzymes for therapeutic drugs. Comput. Biomed. Res., 21: 579-592.

  48. Duggleby, R.G. (1988) Determination of inhibition constants, I50 values and the type of inhibition for enzyme-catalysed reactions. Biochem. Med. Metab. Biol., 40: 204-212. View PDF file

  49. Rzepczyk, C.M., Ramasamy, R., Ho., P.C-L., Mutch, D.A., Anderson, K.L., Duggleby, R.G. , Doran, T.J., Murray, B.J., Irving, D.O., Woodrow, G.C., Parkinson, D., Brabin, B.J. and Alpers, M.P. (1988) Identification of T-epitopes within a potential Plasmodium falciparum vaccine antigen: a study of human lymphocyte responses to repeat and non-repeat regions of Pf155/RESA. J. Immunol., 141: 3197-3202.

  50. Duggleby, R.G. and Nash, J.C. (1989) A single parameter family of adjustments for fitting enzyme kinetic models to progress curve data. Biochem. J., 257: 57-64.

  51. Gray, P.J. and Duggleby, R.G. (1989) Analysis of irreversible enzyme inhibition kinetic data. Biochem. J., 257: 419-424. View PDF file

  52. Duggleby, R.G. and Wood, C. (1989) Analysis of progress curves for enzyme-catalyzed reactions: automatic construction of computer programs for fitting integrated rate equations. Biochem. J., 258: 397-402. [Correction (1990) Biochem. J. 270: 843.]

  53. Duggleby, R.G. and Northrop, D.B. (1989) Quantitative analysis of absorption spectra and application to the characterization of ligand binding curves. Experientia, 45: 87-92. View PDF file

  54. Duggleby, R.G. and Northrop, D.B. (1989) The expression of kinetic isotope effects during the time course of enzyme-catalysed reactions. Bioorg. Chem., 17: 177-193.

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  55. Szedlacsek, S.E., Ostafe, V., Duggleby, R.G., Serban, M. and Vlad, M.O. (1990) Progress curve equations for reversible enzyme-catalysed reactions inhibited by tight-binding inhibitors. Biochem. J., 265: 647-653. View PDF file

  56. Thomas, G., Diefenbach, R. and Duggleby, R.G. (1990) Inactivation of pyruvate decarboxylase by 3-hydroxypyruvate. Biochem. J., 266: 305-308. View PDF file

  57. Duggleby, R.G. (1990) Pooling and comparing estimates from several experiments of a Michaelis constant for an enzyme. Analyt. Biochem., 189: 84-87. View PDF file

  58. Duggleby, R.G. (1990) A computer program in BASIC for calculating gradients used in chromatography, electrophoresis and centrifugation. Comput. Biol. Med., 20: 231-234. View PDF file

  59. Northrop, D.B. and Duggleby, R.G. (1990) Calculations of intrinsic isotope effects and the detection of tunneling in enzyme-catalysed reactions. Bioorg. Chem. 18: 435-439. View PDF file

  60. Duggleby, R.G. (1991) Analysis of biochemical data by nonlinear regression: Is it a waste of time? TIBS, 16: 51-52. View PDF file

  61. Diefenbach, R.J. and Duggleby, R.G. (1991) Pyruvate decarboxylase from Zymomonas mobilis: structure and re-activation of apoenzyme by the cofactors thiamine diphosphate and magnesium ion. Biochem. J., 276: 439-445. View PDF file

  62. Duggleby, R.G. (1991) Determination of the maximum velocity and Michaelis constant of enzymes by a fixed-point method which avoids the necessity to measure initial rates. Biochim. Biophys. Acta, 1078: 124-125. View PDF file

  63. Duggleby, R.G. and Ward, L.C. (1991) Analysis of physiological data characterized by two regimes separated by an abrupt transition. Physiol. Zool., 64: 885-889.

  64. Szedlacsek, S.E., Duggleby, R.G. and Vlad, M.O. (1991) Enzyme catalysis as a chain reaction. Biochem. J., 279: 855-861. View PDF file

  65. Duggleby, R.G. and Clarke, R.B. (1991) Experimental designs for estimating the parameters of the Michaelis-Menten equation from progress curves of enzyme-catalysed reactions. Biochim. Biophys. Acta, 1080: 231-236. View PDF file

  66. Nixon, P.F., and Duggleby, R.G. (1991) The N-terminal amino acid sequence of yeast transketolase. Prot. Seq. Data Anal., 4: 325-326.

  67. Candy, J.M., Duggleby, R.G. and Mattick, J.M. (1991) Expression of active yeast pyruvate decarboxylase in Escherichia coli. J. Gen. Microbiol., 137: 2811-2815.

  68. Duggleby, R.G. (1992) Analysis of fractional turnover rates by exponential regression. Comput. Meth. Prog. Biomed., 37: 65-66. View PDF file

  69. Nixon, P.F., Diefenbach, R.J. and Duggleby, R.G. (1992) Inhibition of transketolase and pyruvate decarboxylase by omeprazole. Biochem. Pharmac., 44: 177-179. View PDF file

  70. Candy, J.M., Diefenbach, R.J., Mattick, J.S. and Duggleby R.G. (1992) Revised sequence for the Zymomonas mobilis pyruvate decarboxylase gene and protein. Life Sci. Adv. Biochem., 11: 59-63.

  71. Diefenbach, R.J., Candy, J.M., Mattick, J.S. and Duggleby, R.G. (1992) Effects of substitution of aspartate-440 and tryptophan-487 in the thiamin diphosphate binding region of pyruvate decarboxylase from Zymomonas mobilis. FEBS Lett., 296: 95-98. View PDF file

  72. Endre, Z.H. and Duggleby, R.G. (1993) Non-linear regression and variance ratio analysis of time-based NMR data. NMR Biomed., 6: 130-135. View PDF file

  73. Waters, M.J. and Duggleby, R.G. (1993) Nonlinear regression analysis of the time course of ligand binding experiments. J. Recept. Res., 13: 1135-1146.

  74. Cross, M., Endre, Z.H., Stewart-Richardson, P., Cowin, G.J., Westhuyzen, J., Duggleby, R.G. and Fleming, S.J. (1993) 23Na-NMR detects hypoxic injury in intact kidney: increases in sodium inhibited by DMSO and DMTU. Magn. Reson. Med., 30: 465-475. View PDF file

  75. Duggleby, R.G. (1994) Calculation of the molecular weight of proteins from electrophoretic and gel exclusion chromatographic experiments. Comput. Appl. Biosci., 10: 133-135.

  76. Duggleby, R.G. (1994) Analysis of progress curve for enzyme-catalysed reactions: application to unstable enzymes, coupled reactions and transient-state kinetics. Biochim. Biophys. Acta, 1205: 268-274. View PDF file

  77. Candy, J.M. and Duggleby, R.G. (1994) Investigation of the cofactor binding site of Zymomonas mobilis pyruvate decarboxylase by site-directed mutagenesis. Biochem. J., 300: 7-13. View PDF file

  78. Endre, Z.H., Cowin, G.J., Stewart-Richardson, P., Cross, M., Willgoss, D.A. and Duggleby, R.G. (1994) 23Na-NMR detects protection by glycine and alanine against hypoxic injury in the isolated perfused rat kidney. Biochem. Biophys. Res. Commun., 202: 1639-1644. View PDF file

  79. Duggleby, R.G. (1994) Product inhibition of reversible enzyme-catalysed reactions. Biochim. Biophys. Acta, 1209: 238-240. View PDF file

  80. Szabados, E., Hindmarsh, E.J., Phillips, L., Duggleby, R.G. and Christopherson, R.I. (1994) 5-Aminoimidazole-4-carboxamide ribotide transformylase-IMP cyclohydrolase from human CCRF-CEM leukemia cells: purification, pH-dependence and inhibitors. Biochemistry, 33: 14237-14245. View PDF file

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  81. Duggleby, R.G. (1995) Analysis of enzyme progress curves by non-linear regression. Meth. Enzymol., 249: 61-90.

  82. Szedlacsek, S.E. and Duggleby, R.G. (1995) Kinetics of slow and tight-binding inhibitors. Meth. Enzymol., 249: 144-180.

  83. Sun, S., Duggleby, R.G. and Schowen, R.L. (1995) The linkage of catalysis and regulation in enzyme action. Carbon isotope effects, solvent isotope effects and proton inventories for the unregulated pyruvate decarboxylase of Zymomonas mobilis. J. Amer. Chem. Soc., 117: 7317-7322. View PDF file or view supporting information


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  84. Duggleby, R.G. , Chao, Y.C., Huang, J.G., Peng, H.L. and Chang, H.Y. (1996) Sequence differences between human muscle and liver cDNAs for UDPglucose pyrophosphorylase and kinetic differences of the recombinant enzymes expressed in Escherichia coli. Eur. J. Biochem., 235: 173-179. View PDF file.

  85. Chang, H.Y., Peng, H.L., Chao, Y.C. and Duggleby, R.G. (1996) The importance of conserved residues in human liver UDPglucose pyrophosphorylase. Eur. J. Biochem., 236: 723-728. View PDF file.

  86. Candy, J.M., Koga, J., Nixon, P.F. and Duggleby, R.G. (1996) The role of residues glutamate-50 and phenylalanine-496 in Zymomonas mobilis pyruvate decarboxylase. Biochem. J., 315: 745-751. View PDF file

  87. Duggleby, R.G. , Peng, H.L. and Chang, H.Y. (1996) An improved assay for UDPglucose pyrophosphorylase and other enzymes that have nucleotide products. Experientia, 52: 568-572. View PDF file.

  88. Candy, J.M., Nixon, P.F., England, R., Schenk, G., Koga, J. and Duggleby, R.G. (1996) Site-directed mutagenesis of E50, F496 and H113 in Zymomonas mobilis pyruvate decarboxylase. In Biochemistry and Physiology of Thiamin Diphosphate Enzymes. H. Bisswanger and A. Schellenberger, eds., A. and C. Intemann, Wissenschaftlicher Verlag, Prien, Germany: 82-102.

  89. Schloss, J.V., Hixon, M.S., Chu, F., Chang, S. and Duggleby, R.G. (1996) Products formed in the oxygen-consuming reactions of acetolactate synthase and pyruvate decarboxylase. In Biochemistry and Physiology of Thiamin Diphosphate Enzymes. H. Bisswanger and A. Schellenberger, eds., A. and C. Intemann, Wissenschaftlicher Verlag, Prien, Germany: 580-585.

  90. Szabados, E., Duggleby, R.G. and Christopherson, R.I. (1996) Metabolism of adenosine and deoxyadenosine by human erythrocytes and CCRF-CEM leukemia cells. Int. J. Biochem. Cell Biol., 28: 1405-1414. View PDF file.


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  91. Varón, R., García-Sevilla, F., García-Moreno, M., García-Cánovas, F., Peyró, R and Duggleby, R.G. (1997) Computer program for the equations describing the steady-state of enzyme reactions. Comput. Appl. Biosci., 13: 159-167.

  92. Schenk, G., Layfield, R., Candy, J.M., Duggleby, R.G. and Nixon, P.F. (1997) Molecular evolutionary analysis of the thiamin-dependent enzyme transketolase. J. Molec. Evol., 44: 552-572. View PDF file.

  93. Duggleby, R.G. (1997) Identification of an acetolactate synthase small subunit gene in two eucaryotes. Gene, 190: 245-249. View PDF file

  94. Chang, A.K. and Duggleby, R.G. (1997) Expression, purification and characterization of Arabidopsis thaliana acetohydroxyacid synthase. Biochem. J., 327: 161-169. View PDF file

  95. Schenk, G., Leeper, F.J., England, R., Nixon, P.F. and Duggleby, R.G. (1997) The role of His113 and His114 in pyruvate decarboxylase from Zymomonas mobilis. Eur. J. Biochem., 248: 63-71. View PDF file.

  96. Hill, C.M., Pang, S.S. and Duggleby, R.G. (1997) Purification of Escherichia coli acetohydroxyacid synthase isozyme II and reconstitution of active enzyme rom its individual pure subunits. Biochem. J., 327: 891-898. View PDF file


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  97. Schenk, G., Duggleby, R.G. and Nixon, P.F. (1998) Heterologous expression of human transketolase. Int. J. Biochem. Cell. Biol., 30: 369-378. View PDF file

  98. Candy, J. M. and Duggleby, R.G. (1998) Structure and properties of pyruvate decarboxylase and site-directed mutagenesis of the Zymomonas mobilis enzyme. Biochim. Biophys. Acta, 1385: 323-338. View PDF file

  99. Chang, A.K. and Duggleby, R.G. (1998) Herbicide-resistant forms of Arabidopsis thaliana acetohydroxyacid synthase: characterization of the catalytic properties and sensitivity to inhibitors of four defined mutants. Biochem. J., 333: 765-777. View PDF file

  100. Hill, C.M. and Duggleby, R.G. (1998) Mutagenesis of Escherichia coli acetohydroxyacid synthase isoenzyme II and characterization of three herbicide-insensitive forms. Biochem. J., 335: 653-661. View PDF file

  101. Fang, R., Nixon, P.F. and Duggleby, R.G. (1998) Identification of the catalytic glutamate in the E1 component of human pyruvate dehydrogenase. FEBS Lett., 437: 273-277. View PDF file

  102. Schenk, G., Duggleby, R.G. and Nixon, P.F. (1998) Properties and functions of the thiamin diphosphate dependent enzyme transketolase. Int. J. Biochem. Cell. Biol., 30: 1297-1318. View PDF file


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  103. Goudar, C.T., Sonnad, J.R. and Duggleby, R.G. (1999) Parameter estimation using a direct solution of the integrated Michaelis-Menten equation. Biochim. Biophys. Acta, 1429: 377-383.

  104. Hill, C.M. and Duggleby, R.G. (1999) Purified recombinant Escherichia coli ketol-acid reductoisomerase is unsuitable for use in a coupled assay of acetohydroxyacid synthase activity due to an unexpected side reaction. Protein Expression Purif., 15: 57-61. View PDF file

  105. Brix, L.A., Duggleby, R.G., Gaedigk, A. and McManus, M.E. (1999) Structural characterisation and functional analysis of human aryl sulfotransferases. Biochem. J., 337: 337-343. View PDF file.

  106. Huang, C.-Y., Nixon P.F. and Duggleby, R.G. (1999) Effect of mutagenesis of V111 and L112 on the substrate specificity of Zymomonas mobilis pyruvate decarboxylase. J. Biochem. Molec. Biol., 32: 39-44. View PDF file

  107. Chang, A.K., Nixon P.F. and Duggleby, R.G. (1999) Aspartate-27 and glutamate-473 are involved in catalysis by Zymomonas mobilis pyruvate decarboxylase. Biochem. J., 339: 255-260. View PDF file.

  108. Pang, S.S. and Duggleby, R.G. (1999) Expression, purification, characterization and reconstitution of the large and small subunits of yeast acetohydroxyacid synthase. Biochemistry, 38: 5222-5231. View PDF file

  109. Lee, Y.-T., Chang, A.K. and Duggleby, R.G. (1999) Effect of mutagenesis at serine 653 of Arabidopsis thaliana acetohydroxyacid synthase on the sensitivity to imidazolinone and sulfonylurea herbicides. FEBS Lett., 452: 341-345. View PDF file

  110. Brix, L.A., Lonie, A., Duggleby, R.G. and McManus, M.E. (1999) Functional studies of amino acid 146 in human aryl sulfotransferases. Biochemistry, 38: 10474-10479. View PDF file


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  111. García-Sevilla, F., Garrido-del Solo, C., Duggleby, R.G. , García-Cánovas, F., Peyró, R. and Varón, R. (2000) Use of a Windows program for simulation of the progress curves of reactants and intermediates involved in enzyme-catalyzed reactions. BioSystems., 54: 151-164. View PDF file.

  112. Duggleby, R.G. and Pang, S.S. (2000) Acetohydroxyacid synthase. J. Biochem. Molec. Biol., 33: 1-36. View PDF file.

  113. Duggleby, R.G., Kartikasari, A.E.R., Wunsch, R.M., Lee. Y.-T., Kil, M.-W., Shin, J.-Y. and Chang, S.-I. (2000) Expression in Escherichia coli of a putative human acetohydroxyacid synthase. J. Biochem. Molec. Biol., 33: 195-201. View PDF file

  114. Lee. Y.-T. and Duggleby, R.G. (2000) Mutagenesis studies on the sensitivity of Escherichia coli acetohydroxyacid synthase II to herbicides and valine. Biochem. J., 350: 69-73. View PDF file.

  115. Chang, A.K., Nixon, P.F. and Duggleby, R.G. (2000) Effects of deletions at the carboxyl terminus of Zymomonas mobilis pyruvate decarboxylase on the kinetic properties and substrate specificity. Biochemistry, 39: 9430-9437. View PDF file

  116. Wu, Y.-G., Chang, A.K., Nixon, P.F., Li, W. and Duggleby, R.G. (2000) Mutagenesis at Asp-27 of pyruvate decarboxylase from Zymomonas mobilis: effect on its ability to form acetoin and acetolactate. Eur. J. Biochem., 267: 6493-6500. View PDF file


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  117. Wu, Y.-G., Widjaja, S.L., Huang, C.-Y., Li, W., Nixon, P.F. and Duggleby, R.G. (2001) Congenital lactic acidosis: evaluation of the properties of the A199T natural variant of human pyruvate dehydrogenase E1a by in vitro mutation. Molec. Genet. Metab., 72: 269-272. View PDF file

  118. Dumas, R., Biou, V., Halgand, F., Douce, R. and Duggleby, R.G. (2001) Enzymology, structure and dynamics of acetohydroxy acid isomeroreductase. Accounts Chem. Res., 34: 399-408. View PDF file

  119. Lee, Y.-T. and Duggleby, R.G. (2001) Identification of the regulatory subunit of Arabidopsis thaliana acetohydroxyacid synthase and reconstitution with its catalytic subunit. Biochemistry, 40: 6836-6844. View PDF file

  120. Duggleby, R.G. (2001) Quantitative analysis of the time courses of enzyme-catalyzed reactions. Methods, 24: 168-174. View PDF file

  121. Huang, C.-Y., Chang, A.K., Nixon P.F. and Duggleby, R.G. (2001) Site-directed mutagenesis of the ionizable groups in the active site of Zymomonas mobilis pyruvate decarboxylase: effect on activity and pH dependence. Eur. J. Biochem., 268: 3558-3565. View PDF file

  122. Pang, S.S. and Duggleby, R.G. (2001) Regulation of yeast acetohydroxyacid synthase by valine and ATP. Biochem. J., 357: 749-757. View PDF file.

  123. Pang, S.S., Guddat, L.W. and Duggleby, R.G. (2001) Crystallization of the catalytic subunit of Saccharomyces cerevisiae acetohydroxyacid synthase. Acta Cryst., D57: 1321-1323. View PDF file.


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  124. Lee, Y.-T. and Duggleby, R.G. (2002) Regulatory interactions in Arabidopsis thaliana acetohydroxyacid synthase. FEBS Lett., 512: 180-184. View PDF file.

  125. Pang, S.S., Duggleby, R.G. and Guddat, L.W. (2002) Crystal structure of yeast acetohydroxyacid synthase: a target for herbicidal inhibitors. J. Molec. Biol., 317: 249-262. View PDF file.

  126. Pang, S.S., Guddat, L.W. and Duggleby, R.G. (2002) Crystallization of the FAD-independent acetolactate synthase of Klebsiella pneumoniae. Acta Cryst., D58: 1237-1239. View PDF file.

  127. Pang, S.S., Guddat, L.W. and Duggleby, R.G. (2002) Yeast acetohydroxyacid synthase. Crystal structure of an enzyme containing non-catalytic FAD. In Flavins and Flavoproteins 2002. S. Chapman, R. Perham and N. Scrutton, eds., Rudolf Weber; Berlin, Germany: 63-68. View PDF file.


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  128. Pang, S.S., Guddat, L.W. and Duggleby, R.G. (2003). Molecular basis of sulfonylurea herbicide inhibition of acetohydroxyacid synthase. J. Biol. Chem., 278: 7639-7644. View PDF file.

  129. Gamage, N.U., Duggleby, R.G., Barnett, A.C., Tresillian, M., Latham, C.F., Liyou, N.E., McManus, M.E. and Martin, J.L. (2003). Structure of a human carcinogen converting enzyme, SULT1A1: structural and kinetic implications of substrate inhibition. J. Biol. Chem., 278: 7655-7662. View PDF file.

  130. Duggleby, R.G., Pang, S.S., Yu, H. and Guddat, L.W. (2003). Systematic characterization of mutations in yeast acetohydroxyacid synthase: Interpretation of herbicide-resistance data. Eur. J. Biochem., 270: 2895-2904. View PDF file.

  131. Tittmann, K., Golbik, R., Uhlemann, K., Khailova, L., Schneider, G., Patel, M., Jordan, F., Chipman, D.M., Duggleby, R.G., and Hübner, G. (2003) NMR Analysis of Covalent Intermediates in Thiamin Diphosphate Enzymes. Biochemistry, 42: 7885-7891. View PDF file

  132. Wu, Y.-G., Chen, W.-Y., Zhang, Z-W., Yang, G.-Z., Li, W. and Duggleby, R.G. (2003) Biochemical characterization of two mutants of human pyruvate dehydrogenase, F205L and T231A of the E1a subunit. J. Inherit. Metab. Dis., 26: 671-674. View PDF file.


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  133. Tittmann, K., Golbik, R., Uhlemann, K., Khailova, L., Patel, M., Jordan, F., Chipman, D., Duggleby, R.G. and Hübner G. (2004) How Thiamine Works in Enzymes: Time-Resolved NMR Snapshots of ThDP-Dependent Enzymes in Action. In Thiamine: Catalytic Mechanisms in Normal and Disease States. F. Jordan and M.S. Patel, eds., Marcel Dekker, Inc. New York, USA, 57-76.

  134. Duggleby, R.G., Guddat, L.W. and Pang, S.S. (2004) Structure and properties of acetohydroxyacid synthase. In Thiamine: Catalytic Mechanisms in Normal and Disease States. F. Jordan and M.S. Patel, eds., Marcel Dekker, Inc. New York, USA, 251-274.

  135. Pang, S.S., Guddat, L.W. and Duggleby, R.G. (2004) Crystallization of Arabidopsis thaliana acetohydroxyacid synthase in complex with the sulfonylurea herbicide chlorimuron ethyl. Acta Cryst. D., 60: 153-155. View PDF file.

  136. Pang, S.S., Duggleby, R.G., Schowen, R.L. and Guddat, L.W. (2004) The crystal structures of Klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactor and with an unusual intermediate. J. Biol. Chem., 279: 2242 - 2253. View PDF file.

  137. Tyagi, R, Lai, R. and Duggleby, R.G. (2004) A new approach to 'megaprimer' PCR mutagenesis without an intermediate gel purification step. BMC Biotech., 4: 2.1-2.6. View PDF file.

  138. Barnett, A.C., Tsvetanov, S., Gamage, N., Martin, J.L, Duggleby, R.G. and McManus, M.E. (2004) Active site mutations and substrate inhibition in human SULT1A1 and human SULT1A3. J. Biol. Chem., 279: 18799-18805. View PDF file.

  139. Tittmann, K., Schröder, K., Golbik, R., McCourt, J., Kaplun, A., Duggleby, R.G., Barak, Z., Chipman, D. and Hübner, G. (2004) Electron transfer in acetohydroxy acid synthase as a side reaction of catalysis. Implications for the reactivity and partitioning of the carbanion/enamine form of (a-hydroxyethyl)thiamin diphosphate in a "nonredox" flavoenzyme. Biochemistry, 43: 8652-8661. View PDF file.

  140. McCourt, J.A., Tyagi, R., Guddat, L.W., Biou, V. and Duggleby, R.G. (2004) Facile crystallization of Escherichia coli ketol-acid reductoisomerase. Acta Cryst. D., 60: 1432-1434. View PDF file.


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  141. Tyagi, R., Lee, Y.-T., Guddat, L.W. and Duggleby, R.G. (2005) Probing the active site of the bifunctional enzyme ketol-acid reductoisomerase by site-directed mutagenesis. FEBS J., 272: 593-602. View PDF file.

  142. McCourt, J.A., Pang, S.S., Guddat, L.W. and Duggleby, R.G. (2005) Elucidating the specificity of sulfonylurea herbicide binding to acetohydroxyacid synthase. Biochemistry, 44: 2330-2338. View PDF file.

  143. Lee, Y.-T., Ta, H.T. and Duggleby, R.G. (2005) Cyclopropane-1,1-dicarboxylate is a slow-, tight-binding inhibitor of rice ketol-acid reductoisomerase. Plant. Sci., 168: 1035-1040. View PDF file.

  144. Duggleby, R.G. (2005) Suicide inhibition of acetohydroxyacid synthase by hydroxypyruvate. J. Enzyme Inhib. Med. Chem., 20: 1-4. View PDF file.

  145. Hempel, N., Barnett, A., Gamage, N., Duggleby, R.G., Windmill, K.F., Martin, J.L. and McManus, M.E. (2005) Human SULT1A SULTs. In Human Cytosolic Sulfotransferases. G.M. Pacifici, and M.W.H. Coughtrie, (eds) Taylor and Francis, London, 179-230.

  146. Wang, B.-L., Duggleby, R.G., Li, Z.-M., Wang, J.-G., Li, Y.-H., Wang, S.-H. and Song, H.-B. (2005) Synthesis, crystal structure and herbicidal activity of intermediate mimics of the KARI reaction. Pest Manag. Sci., 61: 407-412. View PDF file.

  147. McCourt, J.A. and Duggleby, R.G. (2005) How an enzyme answers multiple choice questions. TIBS, 30: 222-225. View PDF file.

  148. Reboutier, D., Frankart, C., Vedel, R., Brault, M., Duggleby, R.G., Rona, J.P., Barny, M.A. and Bouteau, F. (2005) A CFTR chloride channel activator prevents HrpNea-induced cell death in Arabidopsis thaliana suspension cells. Plant Physiol. Biochem., 43: 567-573. View PDF file.

  149. Chipman, D.M., Duggleby, R.G. and Tittmann, K. (2005) Mechanisms of acetohydroxyacid synthases. Curr. Opin. Chem. Biol., 9: 475-481. View PDF file.

  150. Tyagi, R., Duquerroy, S., Navaza, J., Guddat, L.W. and Duggleby, R.G. (2005) The crystal structure of a bacterial Class II ketol-acid reductoisomerase: Domain conservation and evolution. Prot. Sci., 14: 3089-3100. View PDF file.

  151. Gamage, N.U, Tsvetanov, S., Duggleby, R.G., McManus, M.E. and Martin, J.L. (2005) The structure of human SULT1A1 crystallized with estradiol: an insight into active site plasticity and substrate inhibition with multi-ring substrates. J. Biol. Chem., 280: 41482-41486. View PDF file.

  152. Wang, J.-G., Li, Z.-M., Ma, N., Wang, B.-L., Jiang, L., Pang, S.S., Lee, Y.-T., Guddat, L.W. and Duggleby, R.G. (2005) Structure-activity relationships for a new family of sulfonylurea herbicides. J. Comput.-Aided Mol. Des., 19: 801-820. View PDF file.


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  153. McCourt, J.A., Pang, S.S., King-Scott, J., Guddat, L.W. and Duggleby, R.G. (2006) Herbicide binding sites revealed in the structure of plant acetohydroxyacid synthase. Proc. Natl. Acad. Sci. USA, 103: 569-573. View PDF file.

  154. Gamage, N., Barnett, A., Hempel, N., Duggleby, R.G., Windmill, K.F., Martin, J.L. and McManus, M.E. (2006) Human sulfotransferases and their role in chemical metabolism. Toxicol. Sci., 90: 5-22. View PDF file.

  155. Lee, Y.-T. and Duggleby, R.G. (2006) Mutations in the regulatory subunit of yeast acetohydroxyacid synthase affect its activation by MgATP. Biochem. J., 395: 331-336. View PDF file.

  156. McCourt, J.A. and Duggleby, R.G. (2006) Acetohydroxyacid synthase and its role in the biosynthetic pathway for branched-chain amino acids. Amino acids, 31: 173-210 View PDF file.

  157. Duggleby, R.G. (2006) Domain relationships in thiamine diphosphate-dependent enzymes. Accounts Chem. Res., 39: 550-557. View PDF file.

  158. McCourt, J.A., Nixon, P.F. and Duggleby, R.G. (2006) Thiamin nutrition and catalysis-induced instability of thiamin diphosphate. Br. J. Nutr., 96: 636-638. View PDF file.

  159. Yago, J.M., García-Sevilla, F., Garrido-del Solo, C., Duggleby, R.G. and Varón, R. (2006) A Windows program for the derivation of steady-state equations in enzyme systems. Appl. Math. Comput., 181: 837-852. View PDF file.

  160. Duggleby, R.G. (2007) Quantifying the inactivation rate constants for the molecular species comprising the catalytic cycle of Leuconostoc mesenteroides glucose 6-phosphate dehydrogenase. J. Enzyme Inhib. Med. Chem., 22: 141-146. View PDF file.

  161. Duggleby, R.G., McCourt, J.A. and Guddat, L.W. (2008) Structure and mechanism of inhibition of plant acetohydroxyacid synthase. Plant Physiol. Biochem., 46 : 309-324. View PDF file.

  162. Wang, J.-G., Lee, P., Dong, Y.-H., Pang, S.-S., Duggleby, R.G. Li, Z.-M. and Guddat, L.W. (2009) Crystal structures of two novel sulfonylurea herbicides in complex with Arabidopsis thaliana acetohydroxyacid synthase. FEBS. J., 276: 1282-1290. View PDF file.

  163. Garcia-Sevilla, F., Arribas, E., Bisswanger, H., Garcia-Moreno, M., Garcia-Canovas, F., Gomez-Ladron de Guevara, R., Duggleby, R.G., Yago, J.M. and Varon, R. (2010) wREFERASS: Rate equations for enzyme reactions at steady state under MS-Windows, Match, 63: 553-571. View PDF file.

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